Recombinant DNA and Polymerase chain reaction

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The complementary strands of DNA can be separated and re-associated by heating and cooling

One strand of DNA specifies the sequence of the other strand

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Polymerase Chain Reaction

Used to make more copies of DNA from a tiny DNA sample

http://www.sumanasinc.com/webcontent/animations/content/pcr.html

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Polymerase Chain Reaction (PCR) Amplifies DNA

Primers specify what DNA is copied

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PCR Amplifies DNA

Diagnosis

Epidemiology

Genetic engineering

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Flow Chart of PCR

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Production of Insulin

Making recombinant vaccinations

Making food crops with immunity to insects

Forensic Crime scene analysis –

DNA profiling

Real World Applications

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Other Issues

Ethical issues related to “cloning” of human genes

How will genetically engineered organisms affect environment?

Spread of genes to other organisms?

Who will decide?

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