Gel Electrophoresis

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Gel electrophoresis

L Mathias

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Definition

Separation of DNA fragments according to size, based on movement through a gel medium when an electric field is applied.

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DNA negatively charged

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DNA cut up using restriction endonucleases

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Make up the gel which the DNA will be put into

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Dye added to the DNA

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Buffer solution added to the tank

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DNA samples loaded into wells

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Electrical current applied to the chamber

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DNA is stained using ethidium bromide