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Gel Electrophoresis
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Slide 1

Gel electrophoresis

Gel electrophoresis

L Mathias

Slide 2

Definition

Definition

Separation of DNA fragments according to size, based on movement through a gel medium when an electric field is applied.

Slide 3

DNA negatively charged

DNA negatively charged

Slide 4

DNA cut up using restriction endonucleases

DNA cut up using restriction endonucleases

Slide 5

DNA negatively charged

Slide 6

Make up the gel which the DNA will be put into

Make up the gel which the DNA will be put into

Slide 7

DNA negatively charged

Slide 8

DNA negatively charged

Slide 9

DNA negatively charged

Slide 10

Dye added to the DNA

Dye added to the DNA

Slide 11

Buffer solution added to the tank

Buffer solution added to the tank

Slide 12

DNA samples loaded into wells

DNA samples loaded into wells

Slide 13

Electrical current applied to the chamber

Electrical current applied to the chamber

Slide 14

DNA is stained using ethidium bromide

DNA is stained using ethidium bromide

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