Free Powerpoint Presentations

PCR
Page
1

DOWNLOAD

PREVIEW

WATCH ALL SLIDES

Slide 1

Polymerase Chain Reaction

Polymerase Chain Reaction

Aims

To understand the process of PCR and its uses.

Starter - Match each term with its correct description (work in pairs)

04 September 2015

Slide 2

What is it?

What is it?

Slide 3

Animations

Animations

http://www.dnalc.org/ddnalc/resources/pcr.html

http://www.maxanim.com/genetics/PCR/pcr.swf

Slide 4

Animations

Slide 5

Animations

Slide 6

Information

Information

Polymerase chain reaction enables large amounts of DNA to be produced from very small samples (0.1ml)

There is a repeating cycle of:

separation of double DNA strands

synthesis of a complementary strand for each

Slide 7

What happens?

What happens?

Sample DNA , nucleotides, DNA primers & thermostable DNA polymerase placed in PCR machine.

Strands of sample DNA separated by heating to 95oC

Mixture cooled to 37oC to allow primers to bind.

Mixture heated to 72oC for replication (optimum temp of DNA polymerase)

Cycle repeats many times (~8mins /cycle)

Slide 8

Problems

Problems

Separation achieved by heating to 95oC no suitable helicase

DNA polymerase cant work on completely single stranded DNA double stranded regions needed at the start of sequence to be copied:

primers (short sequences DNA) complementary to bases at start of region to be copied used

To synthesize primers , base sequence at start must be known

Slide 9

TTAACGGGGCCCTTTAAA .TTTAAACCCGGGTTT

TTAACGGGGCCCTTTAAA .TTTAAACCCGGGTTT

AATTGCCCCGGGAAATTT .AAATTTGGGCCCAAA

the sequence of bases which ONLY flank a particular region of a particular organism's DNA, and NO OTHER ORGANISM'S DNA. This region would be a target sequence for PCR.

Slide 10

The first step for PCR would be to synthesize "primers" of about 20 letters-long

The first step for PCR would be to synthesize "primers" of about 20 letters-long

ONE primer exactly like the lower left-hand sequence, and ONE primer exactly like the upper right-hand sequence:

TTAACGGGGCCCTTTAAA .TTTAAACCCGGGTT

AATTGCCCCGGGAAATTT .>

and:

< TTTAAACCCGGGTTT

AATTGCCCCGGGAAATTT AAATTTGGGCCCAAA

Slide 11

Go to page:
 1  2 

Contents

Last added presentations

2010-2018 powerpoint presentations