Free Powerpoint Presentations

DNA Technology
Page
1

DOWNLOAD

PREVIEW

WATCH ALL SLIDES

Slide 1

DNA Technology

DNA Technology

Slide 2

DNA Extraction

DNA Extraction

Chemical treatments cause cells and nuclei to burst

The DNA is inherently sticky, and can be pulled out of the mixture

This is called spooling DNA

Slide 3

Spooled DNA

Spooled DNA

Slide 4

Cutting DNA

Cutting DNA

Restriction enzymes cut DNA at specific sequences

Useful to divide DNA into manageable fragments

Slide 5

Electrophoresis

Electrophoresis

DNA can be separated based on size and charge

The phosphate groups are negatively charged

DNA is placed in a gel and electricity is run through

Slide 6

Electrophoresis

Electrophoresis

Negative DNA moves toward the positive end

Smaller fragments move farther and faster

Slide 7

Electrophoresis

Electrophoresis

Slide 8

Steps in DNA Sequencing

Steps in DNA Sequencing

Many copies of a single strand of DNA are placed in a test tube

DNA polymerase is added

A mixture of nucleotides is added some of which have dye molecules attached

Each base (A,T,C,G) has a different color dye

Slide 9

Steps in DNA Sequencing

Steps in DNA Sequencing

By chance, some dyed nucleotides & some regular ones are added

Dye molecules are large and stop the chain from growing

Slide 10

DNA Sequencing

DNA Sequencing

The result is DNA fragments of multiple sizes with colors that can be identified

Slide 11

DNA Sequencing

DNA Sequencing

After the gel separates the resulting fragments by size, we 'read' the sequence from bottom to top.

Slide 12

Copying DNA

Copying DNA

Polymerase Chain Reaction

Also called PCR

A method of making many copies of a piece of DNA

Slide 13

Steps in Copying DNA

Steps in Copying DNA

A DNA molecule is placed in a small test tube

DNA polymerase that can work at high temps is added

Slide 14

Go to page:
 1  2  3 

Contents

Last added presentations

2010-2018 powerpoint presentations