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Electron Microscope
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In transmission EM the electrons pass through the specimen

Specimen needs to be extremely thin - 10nm to 100nm

TEM can magnify objects up to 500 000 times

TEM has made it possible to see the details of and discover new organelles - see page 9 in Collins

Slide 10

Transmission Electron Microscope (TEM)

Transmission Electron Microscope (TEM)

Cells or tissues are killed and chemically ‘fixed’ in a complicated and harsh treatment (in full detail in table 3.1 pg 52 Rowland)

How does this differ to light microscopy?

This treatment can result in alterations to the cell - known as artefacts

What will this mean for the images produced?

Slide 11

Transmission Electron Microscope (TEM)

Transmission Electron Microscope (TEM)

Transmission electron micrograph of epithelial cells from a rat small intestine.

Scale bar = 5 mm.

Slide 12

Scanning Electron Microscope (SEM)

Scanning Electron Microscope (SEM)

In Scanning EM microscopes the electrons bounce off the surface of the specimen

Produce images with a three-dimensional appearance

Allow detailed study of surfaces

Slide 13

Scanning Electron Microscope (SEM)

Scanning Electron Microscope (SEM)

Now watch the following clip explaining SEM

Slide 14

Scanning Electron Microscope (SEM)

Scanning Electron Microscope (SEM)

Slide 15

Links

Links

www.learn.co.uk/

www.microscopy-uk.org.uk/intro/index.html

www.mwrn.com/feature/education.asp

http://www.feic.com/support/tem/transmis.htm

http://anka.livstek.lth.se:2080/microscopy/foodmicr.htm

Slide 16

Light & Electron Microscopes

Light & Electron Microscopes

Copy & complete the following table

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