300 nm 400 nm 500 nm 600 nm 700 nm
They can be used as reflectors in two and three color analysis.
They usually do not themselves produce fluorescence.
They are available in short pass versions.
They are excellent as primary barrier filters.
Lower blocking properties
Reduced passing properties
Their reflecting and passing properties are not absolute, this should be considered while dealing with multiple wavelengths
They are inexpensive.
They have very good blocking properties.
They have very good transmission properties.
They can only pass long wavelengths
( hence, can only block short wavelength)
Since they are made of solution of dye and glass, they can themselves produce fluorescence.
Attenuation of the light without discrimination of the wavelength.
N.D filters could be reflective or absorptive type.
They are partially silvered mirrors.
Absorptive N.D filters can not be used here; simply because of the heat, they will melt.
Common cover slips can be used as beamsplitters if small portion of the light is wanted, up to 5%
Filters must be measured at the angle they are going to be used
filters placed at 90o have different properties when they are placed at 45o
Short pass and long pass filters