Slide 9
How do we know which bacteria have the gene?
It is necessary to isolate the host bacteria that contain the gene that has been spliced as only want the recombinant DNA
By having a gene on the same plasmid that gives resistance to an antibiotic, the other bacteria can be removed by culturing the bacteria in a medium that contains the antibiotic.
The bacteria containing the resistance to the antibiotic will survive and the others will be killed by the antibiotic.
Slide 10
Antibiotic resistance gene used to identify recombinant cells
Slide 11
http://www.sumanasinc.com/webcontent/animations/content/plasmidcloning.html
ANIMATION RECOMBINANT DNA
Slide 12
Plasmids will not work as well in eukaryotic organisms like plants and animals
Other methods need to be used to insert the DNA
Viral vectors can be used for animal cells.
The virus can inject their DNA into an animal host cell.
Can we use this technique on all cells?
Slide 13
Gene Gun can be used to insert genes into plant cells
http://www.hort.purdue.edu/hort/courses/HORT250/animations/Gene%20Gun%20Animation/Genegun1.html
GENE GUN
Slide 14
Biotechnologists are Problem Solvers!
Diabetics having reactions to porcine/animal insulin
Wheat crops being attacked by insects
People sick with cystic fibrosis
All these can be fixed by recombinant DNA!!!
On a Flow Chart show the steps involved in making recombinant DNA for a desired gene. From cutting of the gene to the final product (this may involve the delivery method)
Slide 15
Now we have made the gene how do we get lots of copies??
Slide 16
E.Coli Plasmid is cut with the same restriction enzyme used to cut the insulin gene
Insulin gene is cut from a pancreatic cell DNA using a specific restriction enzyme
insulin - Bacterial cells when supplied with required polypeptides or proteins, the colonies will produce insulin
E.g Vaccines- The plamids are isolated from the e.coli cells, the genes are then amplifyed via PCR and used to create inactivated viruses for vaccines
Slide 17